Molecular basis of live-attenuated influenza virus.
Identifieur interne : 000864 ( Main/Exploration ); précédent : 000863; suivant : 000865Molecular basis of live-attenuated influenza virus.
Auteurs : Wen He [République populaire de Chine] ; Wei Wang ; Huamin Han ; Lei Wang ; Ge Zhang ; Bin GaoSource :
- PloS one [ 1932-6203 ] ; 2013.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Animaux, Grippe humaine (), Grippe humaine (virologie), Humains, Infections à Orthomyxoviridae (), Infections à Orthomyxoviridae (virologie), Lignée cellulaire, Mutation, Protéines virales (génétique), RNA replicase (génétique), Régulation de l'expression des gènes viraux, Souris, Souris de lignée C57BL, Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (physiologie), Vaccins antigrippaux (génétique), Vaccins antigrippaux (usage thérapeutique), Vaccins atténués (génétique), Vaccins atténués (usage thérapeutique).
- MESH :
- génétique : ARN viral, Protéines virales, RNA replicase, Sous-type H1N1 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués.
- physiologie : Sous-type H1N1 du virus de la grippe A.
- usage thérapeutique : Vaccins antigrippaux, Vaccins atténués.
- virologie : Grippe humaine, Infections à Orthomyxoviridae.
- Animaux, Grippe humaine, Humains, Infections à Orthomyxoviridae, Lignée cellulaire, Mutation, Régulation de l'expression des gènes viraux, Souris, Souris de lignée C57BL.
English descriptors
- KwdEn :
- Animals, Cell Line, Gene Expression Regulation, Viral, Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (physiology), Influenza Vaccines (genetics), Influenza Vaccines (therapeutic use), Influenza, Human (prevention & control), Influenza, Human (virology), Mice, Mice, Inbred C57BL, Mutation, Orthomyxoviridae Infections (prevention & control), Orthomyxoviridae Infections (virology), RNA Replicase (genetics), RNA, Viral (genetics), Vaccines, Attenuated (genetics), Vaccines, Attenuated (therapeutic use), Viral Proteins (genetics).
- MESH :
- chemical , genetics : Influenza Vaccines, RNA Replicase, RNA, Viral, Vaccines, Attenuated, Viral Proteins.
- genetics : Influenza A Virus, H1N1 Subtype.
- physiology : Influenza A Virus, H1N1 Subtype.
- prevention & control : Influenza, Human, Orthomyxoviridae Infections.
- chemical , therapeutic use : Influenza Vaccines, Vaccines, Attenuated.
- virology : Influenza, Human, Orthomyxoviridae Infections.
- Animals, Cell Line, Gene Expression Regulation, Viral, Humans, Mice, Mice, Inbred C57BL, Mutation.
Abstract
Human influenza is a seasonal disease associated with significant morbidity and mortality. The most effective means for controlling infection and thereby reducing morbidity and mortality is vaccination with a three inactivated influenza virus strains mixture, or by intranasal administration of a group of three different live attenuated influenza vaccine strains. Comparing to the inactivated vaccine, the attenuated live viruses allow better elicitation of a long-lasting and broader immune (humoral and cellular) response that represents a naturally occurring transient infection. The cold-adapted (ca) influenza A/AA/6/60 (H2N2) (AA ca) virus is the backbone for the live attenuated trivalent seasonal influenza vaccine licensed in the United States. Similarly, the influenza A components of live-attenuated vaccines used in Russia have been prepared as reassortants of the cold-adapted (ca) H2N2 viruses, A/Leningrad/134/17/57-ca (Len/17) and A/Leningrad/134/47/57-ca (Len/47) along with virulent epidemic strains. However, the mechanism of temperature-sensitive attenuation is largely elusive. To understand how modification at genetic level of influenza virus would result in attenuation of human influenza virus A/PR/8/34 (H1N1,A/PR8), we investigated the involvement of key mutations in the PB1 and/or PB2 genes in attenuation of influenza virus in vitro and in vivo. We have demonstrated that a few of residues in PB1 and PB2 are critical for the phenotypes of live attenuated, temperature sensitive influenza viruses by minigenome assay and real-time PCR. The information of these mutation loci could be used for elucidation of mechanism of temperature-sensitive attenuation and as a new strategy for influenza vaccine development.
DOI: 10.1371/journal.pone.0060413
PubMed: 23555969
Affiliations:
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Le document en format XML
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(virologie)</term><term>Lignée cellulaire</term><term>Mutation</term><term>Protéines virales (génétique)</term><term>RNA replicase (génétique)</term><term>Régulation de l'expression des gènes viraux</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (physiologie)</term><term>Vaccins antigrippaux (génétique)</term><term>Vaccins antigrippaux (usage thérapeutique)</term><term>Vaccins atténués (génétique)</term><term>Vaccins atténués (usage thérapeutique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Influenza Vaccines</term><term>RNA Replicase</term><term>RNA, Viral</term><term>Vaccines, Attenuated</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Protéines virales</term><term>RNA replicase</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term><term>Infections à 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The most effective means for controlling infection and thereby reducing morbidity and mortality is vaccination with a three inactivated influenza virus strains mixture, or by intranasal administration of a group of three different live attenuated influenza vaccine strains. Comparing to the inactivated vaccine, the attenuated live viruses allow better elicitation of a long-lasting and broader immune (humoral and cellular) response that represents a naturally occurring transient infection. The cold-adapted (ca) influenza A/AA/6/60 (H2N2) (AA ca) virus is the backbone for the live attenuated trivalent seasonal influenza vaccine licensed in the United States. Similarly, the influenza A components of live-attenuated vaccines used in Russia have been prepared as reassortants of the cold-adapted (ca) H2N2 viruses, A/Leningrad/134/17/57-ca (Len/17) and A/Leningrad/134/47/57-ca (Len/47) along with virulent epidemic strains. However, the mechanism of temperature-sensitive attenuation is largely elusive. To understand how modification at genetic level of influenza virus would result in attenuation of human influenza virus A/PR/8/34 (H1N1,A/PR8), we investigated the involvement of key mutations in the PB1 and/or PB2 genes in attenuation of influenza virus in vitro and in vivo. We have demonstrated that a few of residues in PB1 and PB2 are critical for the phenotypes of live attenuated, temperature sensitive influenza viruses by minigenome assay and real-time PCR. The information of these mutation loci could be used for elucidation of mechanism of temperature-sensitive attenuation and as a new strategy for influenza vaccine development.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Gao, Bin" sort="Gao, Bin" uniqKey="Gao B" first="Bin" last="Gao">Bin Gao</name><name sortKey="Han, Huamin" sort="Han, Huamin" uniqKey="Han H" first="Huamin" last="Han">Huamin Han</name><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Zhang, Ge" sort="Zhang, Ge" uniqKey="Zhang G" first="Ge" last="Zhang">Ge Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="He, Wen" sort="He, Wen" uniqKey="He W" first="Wen" last="He">Wen He</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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